Associate Professor in Orthopaedic Surgery
Associate Professor in Cell Biology
Lab site: http://www.thehiltonlab.com/
A long-term interest of the Hilton Laboratory is to uncover the molecular circuitry regulating lineage commitment, proliferation, and differentiation of skeletal stem cells, cartilage cells (chondrocytes), and bone cells (osteoblasts). Dr. Hilton's lab uses genetic and surgical mouse models as well as primary cell culture techniques coupled with molecular biology and biochemistry to answer questions regarding skeletal stem cell self-renewal and differentiation, chondrogenesis, and osteoblastogenesis. The Hilton Lab’s research focus is divided into three overlapping research programs that include: Skeletal Development, Skeletal Disease, and Skeletal Injury, Repair, and Regeneration.
The Hilton Laboratory is affiliated with the Departments of Orthopaedic Surgery and Cell Biology as part of the Duke Orthopaedic Cellular, Developmental, and Genome Laboratories within the Duke University School of Medicine.
The Hilton Lab has recently developed a novel, patent-pending technology for the isolation, maintenance, and expansion of skeletal stem cells for eventual use in orthopaedic regenerative medicine applications.
Current Projects
- In our Skeletal Development program, projects focused on identifying the mechanisms by which several cell signaling factors: inhibit cartilage formation; induce chondrocyte trans-differentiation; promote osteochondroma tumor formation, and regulate digit number and identity
- In our Skeletal Disease program, projects aimed at understanding the mechanisms associated with joint cartilage maintenance and osteoarthritis
- In our Skeletal Injury, Repair, and Regeneration program, projects geared toward elucidating: the cellular and molecular mechanisms of fracture and critical-sized bone defect repair; novel approaches to the isolation, maintenance, and expansion of skeletal stem cells for use in orthopaedic regenerative medicine applications; and the appropriate means for integrating biologics and biomaterials to induce skeletal regeneration and repair
Selected Publications & Grants
Publications
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Leinroth, A. P., Mirando, A. J., Rouse, D., Kobayahsi, Y., Tata, P. R., Rueckert, H. E., … Hilton, M. J. (2022). Identification of distinct non-myogenic skeletal-muscle-resident mesenchymal cell populations. Cell Rep, 39(6), 110785. https://doi.org/10.1016/j.celrep.2022.110785
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Long, J. T., Leinroth, A., Liao, Y., Ren, Y., Mirando, A. J., Nguyen, T., … Hilton, M. J. (2022). Hypertrophic chondrocytes serve as a reservoir for marrow-associated skeletal stem and progenitor cells, osteoblasts, and adipocytes during skeletal development. Elife, 11. https://doi.org/10.7554/eLife.76932
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Brozowski, J. M., Timoshchenko, R. G., Serafin, D. S., Allyn, B., Koontz, J., Rabjohns, E. M., … Tarrant, T. K. (2022). G protein-coupled receptor kinase 3 modulates mesenchymal stem cell proliferation and differentiation through sphingosine-1-phosphate receptor regulation. In Stem Cell Res Ther (Vol. 13, p. 37). England. https://doi.org/10.1186/s13287-022-02715-4
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Sharma, D., Mirando, A. J., Leinroth, A., Long, J. T., Karner, C. M., & Hilton, M. J. (2021). HES1 is a novel downstream modifier of the SHH-GLI3 Axis in the development of preaxial polydactyly. Plos Genet, 17(12), e1009982. https://doi.org/10.1371/journal.pgen.1009982
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Abar, B., Kelly, C., Pham, A., Allen, N., Barber, H., Kelly, A., … Adams, S. B. (2021). Effect of surface topography on in vitro osteoblast function and mechanical performance of 3D printed titanium. J Biomed Mater Res A, 109(10), 1792–1802. https://doi.org/10.1002/jbm.a.37172
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Wang, K., Donnelly, C. R., Jiang, C., Liao, Y., Luo, X., Tao, X., … Ji, R.-R. (2021). STING suppresses bone cancer pain via immune and neuronal modulation. Nat Commun, 12(1), 4558. https://doi.org/10.1038/s41467-021-24867-2
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Lee, C.-L., Brock, K. D., Hasapis, S., Zhang, D., Sibley, A. B., Qin, X., … Kirsch, D. G. (2021). Whole-Exome Sequencing of Radiation-Induced Thymic Lymphoma in Mouse Models Identifies Notch1 Activation as a Driver of p53 Wild-Type Lymphoma. Cancer Res, 81(14), 3777–3790. https://doi.org/10.1158/0008-5472.CAN-20-2823
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Guo, W., Spiller, K. V., Tang, J., Karner, C. M., Hilton, M. J., & Wu, C. (2021). Hypoxia depletes contaminating CD45+ hematopoietic cells from murine bone marrow stromal cell (BMSC) cultures: Methods for BMSC culture purification. Stem Cell Res, 53, 102317. https://doi.org/10.1016/j.scr.2021.102317
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Roston, R. A., Mirando, A. J., McLellan, W. A., Pabst, D. A., Hilton, M. J., & Roth, V. L. (2021). Sutural structure in a telescoped skull: the maxillo-frontal suture in Tursiops truncatus. In Integrative and Comparative Biology (Vol. 61, pp. E764–E764).
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Wang, K., Donnelly, C., Jiang, C., Liao, Y., Tao, X., Bang, S., … Ji, R.-R. (2021). STING suppresses cancer pain via immune and neuronal modulation. https://doi.org/10.1101/2021.01.18.426944
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Grants
- Training Program in Developmental and Stem Cell Biology (Mentor), awarded by National Institutes of Health, administered by Basic Science Departments, 2001-2027 More info
- Resorbable, Phsophorylated Poly(ester urea) Surgical Adhesive to Enhance Fracture Healing (Advisor), awarded by National Institutes of Health, administered by Trauma, Acute, and Critical Care Surgery, 2021-2026 More info
- Cell and Molecular Biology Training Program (Mentor), awarded by National Institutes of Health, administered by Basic Science Departments, 2021-2026 More info
- Genetic and Genomics Training Grant (Mentor), awarded by National Institutes of Health, administered by Basic Science Departments, 2020-2025 More info
- Notch Signaling in Endochondral Bone Development (Principal Investigator), awarded by National Institutes of Health, administered by Orthopaedics, 2018-2023 More info
- Notch Signaling in non-myogenic mesenchymal cells regulates muscle development (Principal Investigator), awarded by National Institutes of Health, administered by Orthopaedics, 2020-2023 More info
- Duke CTSA (TL1) Year 5 (Mentor), awarded by National Institutes of Health, administered by Institutes and Centers, 2018-2023 More info
- Notch Signaling in Joint Cartilage Maintenance and Arthritis (Principal Investigator), awarded by National Institutes of Health, administered by Orthopaedics, 2012-2022 More info
- Zeiss Lightsheet Upgrade to Expand Imaging Capabilities (Minor User), awarded by National Institutes of Health, administered by Biology, 2021-2022 More info
- CaMKK2 Signaling in Osteoarthritis (Principal Investigator), awarded by Indiana University, administered by Orthopaedics, 2020-2022 More info
People
- Matthew J. Hilton, PhD, Principal Investigator, Duke Departments of Orthopaedic Surgery and Cell Biology
- Anthony J. Mirando, MS, Laboratory Manager, Duke Department of Orthopaedic Surgery
- Yinshi Ren, PhD, Postdoctoral Researcher, Duke Department of Orthopaedic Surgery
- Deepika Sharma, MS, Graduate Student, University of Rochester, Department of Biomedical Genetics
- Jason Long, Graduate Student, Duke Department of Cell Biology, CMB Graduate Program
- Abigail Leinroth, Graduate Student, Duke Department of Cell Biology, DSCB Graduate Program
Jobs
Various positions are available in the Hilton Lab for postdoctoral researchers, graduate/medical students, and technicians interested in skeletal development, disease, and repair research. Postdoctoral and technician candidates should provide a cover letter, CV, and contact information for three professional references. Duke graduate and/or medical students should send an email stating your interest in a research rotation. Send to: Matthew J. Hilton, PhD, matthew.hilton@duke.edu
Contact
Levine Science Research Center, 450 Research Drive, Room B321c
DUMC 91009, Durham, NC 27710
919-613-9752
matthew.hilton@duke.edu